ELG1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RTT106
Gene Ontology Biological Process
- DNA replication-dependent nucleosome assembly [IGI]
- DNA replication-independent nucleosome assembly [IMP]
- heterochromatin assembly involved in chromatin silencing [IGI, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI, IMP]
Gene Ontology Molecular Function
Dosage Rescue
A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.
Publication
A genetic screen for high copy number suppressors of the synthetic lethality between elg1Δ and srs2Δ in yeast.
Elg1 and Srs2 are two proteins involved in maintaining genome stability in yeast. After DNA damage, the homotrimeric clamp PCNA, which provides stability and processivity to DNA polymerases and serves as a docking platform for DNA repair enzymes, undergoes modification by the ubiquitin-like molecule SUMO. PCNA SUMOylation helps recruit Srs2 and Elg1 to the replication fork. In the absence of ... [more]
Throughput
- Low Throughput
Additional Notes
- high-copy suppressor of elg1 srs2 double mutants (sectoring screen)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ELG1 RTT106 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| ELG1 RTT106 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| ELG1 RTT106 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 2388479 | |
| RTT106 ELG1 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | High | - | BioGRID | 330128 |
Curated By
- BioGRID