BAIT

ELG1

RTT110, S000007438, YOR144C
Subunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication
Saccharomyces cerevisiae (S288c)
PREY

PPS1

tyrosine/serine/threonine protein phosphatase PPS1, L000003522, YBR276C
Protein phosphatase; has specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle
Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

A genetic screen for high copy number suppressors of the synthetic lethality between elg1Δ and srs2Δ in yeast.

Gazy I, Liefshitz B, Bronstein A, Parnas O, Atias N, Sharan R, Kupiec M

Elg1 and Srs2 are two proteins involved in maintaining genome stability in yeast. After DNA damage, the homotrimeric clamp PCNA, which provides stability and processivity to DNA polymerases and serves as a docking platform for DNA repair enzymes, undergoes modification by the ubiquitin-like molecule SUMO. PCNA SUMOylation helps recruit Srs2 and Elg1 to the replication fork. In the absence of ... [more]

G3 (Bethesda) May. 01, 2013; 3(5);917-26 [Pubmed: 23704284]

Throughput

  • Low Throughput

Additional Notes

  • high-copy suppressor of elg1 srs2 double mutants (sectoring screen)

Curated By

  • BioGRID