BAIT

CBC2

CBP20, MUD13, SAE1, L000002891, L000004111, YPL178W
Small subunit of the heterodimeric cap binding complex with Sto1p; interacts with Npl3p, possibly to package mRNA for export from the nucleus; may have a role in telomere maintenance; contains an RNA-binding motif
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SPP382

CCF8, NTR1, YLR424W
Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Sumoylation of the THO complex regulates the biogenesis of a subset of mRNPs.

Bretes H, Rouviere JO, Leger T, Oeffinger M, Devaux F, Doye V, Palancade B

Assembly of messenger ribonucleoparticles (mRNPs) is a pivotal step in gene expression, but only a few molecular mechanisms contributing to its regulation have been described. Here, through a comprehensive proteomic survey of mRNP assembly, we demonstrate that the SUMO pathway specifically controls the association of the THO complex with mRNPs. We further show that the THO complex, a key player ... [more]

Nucleic Acids Res. Apr. 01, 2014; 42(8);5043-58 [Pubmed: 24500206]

Throughput

  • High Throughput

Additional Notes

  • Table S1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SPP382 CBC2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.258BioGRID
2003591

Curated By

  • BioGRID