BAIT

SPTA1

EL2, HPP, HS3, SPH3, SPTA
spectrin, alpha, erythrocytic 1
Glioblastoma Project
Homo sapiens
PREY

ANK1

ANK, SPH1, SPH2
ankyrin 1, erythrocytic
Homo sapiens

Biochemical Activity (Ubiquitination)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Spectrin's E2/E3 ubiquitin conjugating/ligating activity is diminished in sickle cells.

Chang TL, Kakhniashvili DG, Goodman SR

Erythrocyte spectrin contains E2/E3 ubiquitin conjugating/ligating activity in its alpha subunit. Ankyrin is a target of spectrin's E2/E3 ubiquitin conjugating/ligating activity in vitro and in vivo. We compare the ubiquitination levels of ankyrin mediated by control and sickle cell spectrin using a biotinylated ubiquitin cell-free assay. Sickle cell spectrin has diminished ability to transfer ubiquitin from an intermediate spectrin-ubiquitin thioester ... [more]

Am. J. Hematol. Jun. 01, 2005; 79(2);89-96 [Pubmed: 15929114]

Throughput

  • Low Throughput

Additional Notes

  • in the presence of purified red blood cell inside out vesicles

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SPTA1 ANK1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID