BAIT

SEM1

DSS1, HOD1, proteasome regulatory particle lid subunit SEM1, L000003539, L000004647, YDR363W-A
Component of lid subcomplex of 26S proteasome regulatory subunit; involved in mRNA export mediated by TREX-2 complex (Sac3p-Thp1p); assumes different conformations in different contexts, functions as molecular glue stabilizing the Rpn3p/Rpn7p regulatory heterodimer, and tethers it to lid helical bundle; ortholog of human DSS1; protein abundance increases in response to DNA replication stress
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

RAD16

PSO5, DNA repair protein RAD16, L000004121, L000001565, YBR114W
Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
UPS Project
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Nucleotide excision repair and the 26S proteasome function together to promote trinucleotide repeat expansions.

Concannon C, Lahue RS

Trinucleotide repeat (TNR) expansion underpins a number of inheritable neurological human disorders. Multiple mechanisms are thought to contribute to the expansion process. The incorrect processing of the repeat tract by DNA repair proteins can drive this mutation process forward, as expansions are suppressed following ablation of certain repair factors in mouse models and cell models of disease. Nucleotide excision repair ... [more]

DNA Repair (Amst.) Jan. 01, 2014; 13(0);42-9 [Pubmed: 24359926]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: uv resistance (APO:0000085)

Additional Notes

  • sem1 rad16 double mutant more sensitive to UV than single mutants

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RAD16 SEM1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1316BioGRID
2081265
SEM1 RAD16
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
1036246

Curated By

  • BioGRID