BAIT

FUB1

YCR076C

Proteasome-binding protein; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene

GO Process (0)

GO Function (1)

GO Component (0)

Gene Ontology Molecular Function

proteasome binding [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

PUP2

DOA5, proteasome core particle subunit alpha 5, L000001531, YGR253C

Alpha 5 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit zeta

UPS Project

GO Process (2)

GO Function (0)

GO Component (5)

Gene Ontology Biological Process

proteasomal ubiquitin-independent protein catabolic process [IDA]

proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]

Gene Ontology Cellular Component

cytoplasm [IC]

nuclear outer membrane-endoplasmic reticulum membrane network [IC]

nucleus [IC]

proteasome core complex, alpha-subunit complex [IDA]

proteasome storage granule [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

N-terminal Î±7 deletion of the proteasome 20S core particle substitutes for yeast PI31 function.

Yashiroda H, Toda Y, Otsu S, Takagi K, Mizushima T, Murata S

The proteasome core particle (CP) is a conserved protease complex that is formed by the stacking of two outer Î±-rings and two inner Î²-rings. The Î±-ring is a heteroheptameric ring of Î±1-Î±7 subunits and acts as a gate that restricts entry of substrate proteins into the catalytic cavity formed by the two abutting Î²-rings. Proteasome inhibitor 31-kDa (PI31) was originally ... [more]

Mol. Cell. Biol. Oct. 20, 2014; 0(0); [Pubmed: 25332237]

Throughput

Low Throughput

Additional Notes

Figure 2

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PUP2 FUB1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	6	BioGRID	3617005
PUP2 FUB1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.4259	BioGRID	1986712

Curated By

BioGRID

Interaction Summary