BAIT

POC4

DMP1, PBA4, YPL144W
Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
UPS Project
GO Process (2)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

PRE10

proteasome core particle subunit alpha 7, L000003146, YOR362C
Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
UPS Project
Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

N-terminal α7 deletion of the proteasome 20S core particle substitutes for yeast PI31 function.

Yashiroda H, Toda Y, Otsu S, Takagi K, Mizushima T, Murata S

The proteasome core particle (CP) is a conserved protease complex that is formed by the stacking of two outer α-rings and two inner β-rings. The α-ring is a heteroheptameric ring of α1-α7 subunits and acts as a gate that restricts entry of substrate proteins into the catalytic cavity formed by the two abutting β-rings. Proteasome inhibitor 31-kDa (PI31) was originally ... [more]

Mol. Cell. Biol. Oct. 20, 2014; 0(0); [Pubmed: 25332237]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • Figure 8
  • PRE10 mutation (alpha 7 N-terminal deletion) suppresses lethality of fub1 pba4 double null mutant
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
POC4 PRE10
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
PRE10 POC4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1867BioGRID
2019891

Curated By

  • BioGRID