BAIT

PRE1

proteasome core particle subunit beta 4, L000001483, YER012W
Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

FUB1

YCR076C
Proteasome-binding protein; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

N-terminal α7 deletion of the proteasome 20S core particle substitutes for yeast PI31 function.

Yashiroda H, Toda Y, Otsu S, Takagi K, Mizushima T, Murata S

The proteasome core particle (CP) is a conserved protease complex that is formed by the stacking of two outer α-rings and two inner β-rings. The α-ring is a heteroheptameric ring of α1-α7 subunits and acts as a gate that restricts entry of substrate proteins into the catalytic cavity formed by the two abutting β-rings. Proteasome inhibitor 31-kDa (PI31) was originally ... [more]

Mol. Cell. Biol. Oct. 20, 2014; 0(0); [Pubmed: 25332237]

Throughput

  • Low Throughput

Additional Notes

  • Figure 9

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FUB1 PRE1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High2BioGRID
3615764
PRE1 FUB1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1331BioGRID
1975845

Curated By

  • BioGRID