PREY

ATP6V0B

2310024H13Rik, Atp6f, VMA16, RP23-133J9.4
ATPase, H+ transporting, lysosomal V0 subunit B
GO Process (2)
GO Function (1)
GO Component (3)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interferon-inducible transmembrane proteins of the innate immune response act as membrane organizers by influencing clathrin and v-ATPase localization and function.

Wee YS, Roundy KM, Weis JJ, Weis JH

The innate response interferon-inducible transmembrane (Ifitm) proteins have been characterized as influencing proliferation, signaling complexes and restricting virus infections. Treatment of cells lacking these proteins (IfitmDel) with IFN-β resulted in the loss of clathrin from membrane compartments and the inhibition of clathrin-mediated phagocytosis, suggesting a molecular interaction between clathrin and Ifitm proteins. The pH of endosomes of IfitmDel cells, with ... [more]

Innate Immun Dec. 01, 2012; 18(6);834-45 [Pubmed: 22467717]

Throughput

  • Low Throughput

Curated By

  • BioGRID