BAIT

AHR

bHLHe76
aryl hydrocarbon receptor
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

G-protein signalling negatively regulates the stability of aryl hydrocarbon receptor.

Nakata A, Urano D, Fujii-Kuriyama Y, Mizuno N, Tago K, Itoh H

Aryl hydrocarbon receptor (AhR) is a transcription factor that works as a dioxin receptor and is also involved in various physiological phenomena, including development and cell proliferation. Here, we show that the Galpha13 signal destabilizes AhR by promoting the ubiquitination of AhR. Galpha13 interacts directly with AhR-interacting protein (AIP) and inhibits the interaction between AhR and AIP, a crucial interacting ... [more]

EMBO Rep. Jun. 01, 2009; 10(6);622-8 [Pubmed: 19390533]

Throughput

  • Low Throughput

Curated By

  • BioGRID