UBE2I
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- cellular protein modification process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- post-translational protein modification [TAS]
- protein sumoylation [IDA, TAS]
- protein ubiquitination [IBA]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
XBP1
Gene Ontology Biological Process
- activation of signaling protein activity involved in unfolded protein response [TAS]
- adipose tissue development [ISS]
- angiogenesis [ISS]
- cell growth [IDA]
- cellular protein metabolic process [TAS]
- cellular response to amino acid stimulus [ISS]
- cellular response to fluid shear stress [IDA]
- cellular response to fructose stimulus [ISS]
- cellular response to glucose starvation [ISS]
- cellular response to glucose stimulus [ISS]
- cellular response to insulin stimulus [ISS]
- cellular response to interleukin-4 [ISS]
- cellular response to laminar fluid shear stress [IDA]
- cellular response to lipopolysaccharide [IDA]
- cellular response to nutrient [ISS]
- cellular response to oxidative stress [IDA]
- cellular response to peptide hormone stimulus [ISS]
- cellular response to vascular endothelial growth factor stimulus [IDA]
- cellular triglyceride homeostasis [ISS]
- cholesterol homeostasis [ISS]
- endoplasmic reticulum unfolded protein response [ISS, TAS]
- endothelial cell proliferation [IDA]
- fatty acid homeostasis [ISS]
- immune response [TAS]
- liver development [ISS]
- negative regulation of apoptotic process [ISS]
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [IDA]
- negative regulation of endoplasmic reticulum unfolded protein response [IDA]
- negative regulation of myotube differentiation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- neuron development [ISS]
- phosphatidylinositol 3-kinase signaling [IDA]
- positive regulation of B cell differentiation [IDA]
- positive regulation of MHC class II biosynthetic process [IMP]
- positive regulation of T cell differentiation [IDA]
- positive regulation of TOR signaling [IMP]
- positive regulation of autophagy [IDA]
- positive regulation of endoplasmic reticulum unfolded protein response [IMP]
- positive regulation of endothelial cell apoptotic process [IDA]
- positive regulation of fat cell differentiation [ISS]
- positive regulation of hepatocyte proliferation [ISS]
- positive regulation of histone methylation [IDA]
- positive regulation of immunoglobulin production [IDA]
- positive regulation of immunoglobulin secretion [ISS]
- positive regulation of interleukin-6 secretion [ISS]
- positive regulation of lactation [ISS]
- positive regulation of plasma cell differentiation [IDA]
- positive regulation of protein acetylation [IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of transcription factor import into nucleus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, ISS]
- positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response [IDA, ISS]
- protein destabilization [IDA, IMP]
- regulation of protein stability [IDA]
- response to endoplasmic reticulum stress [IDA]
- response to insulin-like growth factor stimulus [ISS]
- sterol homeostasis [ISS]
- transcription from RNA polymerase II promoter [ISS]
- ubiquitin-dependent protein catabolic process [ISS]
- vascular endothelial growth factor receptor signaling pathway [IDA]
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- chromatin DNA binding [IDA]
- core promoter binding [ISS]
- enhancer sequence-specific DNA binding [IDA]
- protease binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein kinase binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- ubiquitin protein ligase binding [IPI]
- DNA binding [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- chromatin DNA binding [IDA]
- core promoter binding [ISS]
- enhancer sequence-specific DNA binding [IDA]
- protease binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein kinase binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
UBC9 regulates the stability of XBP1, a key transcription factor controlling the ER stress response.
XBP1 is a key transcription factor regulating the mammalian endoplasmic reticulum (ER) stress response, which is a cytoprotective mechanism for dealing with an accumulation of unfolded proteins in the ER (ER stress). The expression of XBP1 is regulated by two different mechanisms: mRNA splicing and protein stability. When ER stress occurs, unspliced XBP1 mRNA is converted to mature mRNA, from ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 4
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| XBP1 UBE2I | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 1036577 | |
| UBE2I XBP1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 1036578 |
Curated By
- BioGRID