BAIT

HRD1

DER3, E3 ubiquitin-protein ligase HRD1, L000002963, YOL013C
Ubiquitin-protein ligase; functions in ER retention of misfolded proteins; required for ER-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

ASI1

putative ubiquitin-protein ligase ASI1, YMR119W
Subunit of the nuclear inner membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by targeting latent unprocessed forms of Stp1p and Stp2p and maintains the repressed state of gene expression in the absence of inducing amino acids; ASI1 has a paralog, ASI3, that arose from the whole genome duplication
UPS Project
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Quality control of inner nuclear membrane proteins by the Asi complex.

Foresti O, Rodriguez-Vaello V, Funaya C, Carvalho P

Misfolded proteins in the endoplasmic reticulum (ER) are eliminated by a quality-control system called ER-associated protein degradation (ERAD). However, how misfolded proteins in the inner nuclear membrane (INM), a specialized ER subdomain, are degraded is not known. Here, a quantitative proteomics approach revealed an ERAD branch required for INM protein quality control in yeast. This branch involved the integral membrane ... [more]

Science Sep. 18, 2014; 0(0); [Pubmed: 25236469]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: protein/peptide accumulation (APO:0000149)

Additional Notes

  • double mutants show increased defects in degradation of sec61

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ASI1 HRD1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
1050599

Curated By

  • BioGRID