BAIT

PMP22

CMT1A, CMT1E, DSS, GAS-3, HMSNIA, HNPP, Sp110
peripheral myelin protein 22
GO Process (4)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

AMFR

GP78, RNF45
autocrine motility factor receptor, E3 ubiquitin protein ligase
UPS Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rer1 and calnexin regulate endoplasmic reticulum retention of a peripheral myelin protein 22 mutant that causes type 1A Charcot-Marie-Tooth disease.

Hara T, Hashimoto Y, Akuzawa T, Hirai R, Kobayashi H, Sato K

Peripheral myelin protein 22 (PMP22) resides in the plasma membrane and is required for myelin formation in the peripheral nervous system. Many PMP22 mutants accumulate in excess in the endoplasmic reticulum (ER) and lead to the inherited neuropathies of Charcot-Marie-Tooth (CMT) disease. However, the mechanism through which PMP22 mutants accumulate in the ER is unknown. Here, we studied the quality ... [more]

Sci Rep Nov. 12, 2014; 4(0);6992 [Pubmed: 25385046]

Throughput

  • Low Throughput

Additional Notes

  • Pmp22 mutants bind Hrd1 and gp78, likely for degradation

Curated By

  • BioGRID