Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Importin alpha1 (Rch1) mediates nuclear translocation of thioredoxin-binding protein-2/vitamin D(3)-up-regulated protein 1.

Nishinaka Y, Masutani H, Oka S, Matsuo Y, Yamaguchi Y, Nishio K, Ishii Y, Yodoi J

Thioredoxin-binding protein-2 (TBP-2)/vitamin D(3) up-regulated protein 1 is an endogenous molecule interacting with thioredoxin (TRX), negatively regulating TRX function, and being implicated in the suppression of tumor development and metastasis. We found that TBP-2 ectopically expressed in the breast cancer cell line MCF-7 was localized predominantly in the nucleus exhibiting growth suppressive activity. The nuclear accumulation of endogenous TBP-2 protein ... [more]

J. Biol. Chem. Sep. 03, 2004; 279(36);37559-65 [Pubmed: 15234975]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TBPL2 KPNA2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Nishinaka Y (2004)	Low	-	BioGRID	-
KPNA2 TBPL2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Nishinaka Y (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TBPL2

Gene Ontology Cellular Component

KPNA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone deacetylase binding [IPI]nuclear localization sequence binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]