BAIT
SHMT2
GLYA, HEL-S-51e, SHMT
serine hydroxymethyltransferase 2 (mitochondrial)
GO Process (1)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
POLA1
NSX, POLA, p180
polymerase (DNA directed), alpha 1, catalytic subunit
GO Process (19)
GO Function (8)
GO Component (8)
Gene Ontology Biological Process
- DNA repair [IDA]
- DNA replication [IMP]
- DNA replication initiation [IDA, TAS]
- DNA replication, synthesis of RNA primer [IDA]
- DNA strand elongation involved in DNA replication [IMP, TAS]
- DNA synthesis involved in DNA repair [IMP]
- G1/S transition of mitotic cell cycle [TAS]
- cell proliferation [IDA]
- double-strand break repair via nonhomologous end joining [IMP]
- lagging strand elongation [IDA]
- leading strand elongation [IDA]
- mitotic cell cycle [TAS]
- nucleic acid phosphodiester bond hydrolysis [IBA]
- nucleotide-excision repair, DNA gap filling [IBA]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [TAS]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
- translesion synthesis [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 ... [more]
Cell Jun. 08, 2012; 149(6);1393-406 [Pubmed: 22658674]
Throughput
- High Throughput
Curated By
- BioGRID