BAIT

ERCC6

4732403I04, C130058G22Rik, CSB
excision repair cross-complementing rodent repair deficiency, complementation group 6
Mus musculus
PREY

POLR1A

194kDa, 3010014K16Rik, RPA194, Rpo1-4, mRPA1
polymerase (RNA) I polypeptide A
GO Process (1)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The chromatin remodeling factor CSB recruits histone acetyltransferase PCAF to rRNA gene promoters in active state for transcription initiation.

Shen M, Zhou T, Xie W, Ling T, Zhu Q, Zong L, Lyu G, Gao Q, Zhang F, Tao W

The promoters of poised rRNA genes (rDNA) are marked by both euchromatic and heterochromatic histone modifications and are associated with two transcription factors, UBF and SL1 that nucleate transcription complex formation. Active rRNA genes contain only euchromatic histone modifications and are loaded with all components of transcriptional initiation complex including RNA polymerase I. Coupled with histone acetylation and RNA polymerase ... [more]

PLoS ONE May. 15, 2013; 8(5);e62668 [Pubmed: 23667505]

Throughput

  • Low Throughput

Additional Notes

  • Figure S2

Curated By

  • BioGRID