KAT2B
Gene Ontology Biological Process
- N-terminal peptidyl-lysine acetylation [IDA]
- Notch signaling pathway [TAS]
- cell cycle arrest [TAS]
- cellular response to insulin stimulus [IDA]
- chromatin organization [TAS]
- chromatin remodeling [IDA, NAS]
- gene expression [TAS]
- histone H3 acetylation [IDA]
- internal peptidyl-lysine acetylation [IDA]
- negative regulation of cell proliferation [IDA]
- peptidyl-lysine acetylation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein acetylation [TAS]
- regulation of protein ADP-ribosylation [IDA]
- transcription from RNA polymerase I promoter [TAS]
- transcription initiation from RNA polymerase I promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- acetyltransferase activity [IDA]
- cyclin-dependent protein serine/threonine kinase inhibitor activity [ISS]
- histone acetyltransferase activity [IDA]
- histone deacetylase binding [IPI]
- lysine N-acetyltransferase activity, acting on acetyl phosphate as donor [IDA, ISS]
- protein binding [IPI]
- protein complex binding [IDA]
- protein kinase binding [ISS]
- transcription coactivator activity [IDA]
- transcription cofactor activity [IPI]
- transcription factor binding [IPI]
- acetyltransferase activity [IDA]
- cyclin-dependent protein serine/threonine kinase inhibitor activity [ISS]
- histone acetyltransferase activity [IDA]
- histone deacetylase binding [IPI]
- lysine N-acetyltransferase activity, acting on acetyl phosphate as donor [IDA, ISS]
- protein binding [IPI]
- protein complex binding [IDA]
- protein kinase binding [ISS]
- transcription coactivator activity [IDA]
- transcription cofactor activity [IPI]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
ACTB
Gene Ontology Biological Process
- 'de novo' posttranslational protein folding [TAS]
- ATP-dependent chromatin remodeling [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- adherens junction organization [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell junction assembly [TAS]
- cell-cell junction organization [TAS]
- cellular component movement [TAS]
- cellular protein metabolic process [TAS]
- chromatin organization [TAS]
- innate immune response [TAS]
- membrane organization [TAS]
- platelet aggregation [IMP]
- protein folding [TAS]
- retina homeostasis [IEP]
- substantia nigra development [IEP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- Tat protein binding [IPI]
- kinesin binding [IPI]
- nitric-oxide synthase binding [IPI]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- structural constituent of cytoskeleton [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- Tat protein binding [IPI]
- kinesin binding [IPI]
- nitric-oxide synthase binding [IPI]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- structural constituent of cytoskeleton [TAS]
Gene Ontology Cellular Component
- MLL5-L complex [IDA]
- NuA4 histone acetyltransferase complex [IDA]
- blood microparticle [IDA]
- cytoplasm [TAS]
- cytoplasmic ribonucleoprotein granule [IDA]
- cytoskeleton [TAS]
- cytosol [TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- nuclear chromatin [IDA]
- nucleoplasm [TAS]
- protein complex [IDA]
- ribonucleoprotein complex [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The histone acetyltransferase PCAF associates with actin and hnRNP U for RNA polymerase II transcription.
Actin is a key regulator of RNA polymerase (pol) II transcription. In complex with specific hnRNPs, it has been proposed that actin functions to recruit pol II coactivators during the elongation of nascent transcripts. Here, we show by affinity chromatography, protein-protein interaction assays, and biochemical fractionation of nuclear extracts that the histone acetyltransferase (HAT) PCAF associates with actin and hnRNP ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 1
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ACTB KAT2B | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1054336 |
Curated By
- BioGRID