An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

p/CAF modulates the activity of the transcription factor p48/Ptf1a involved in pancreatic acinar differentiation.

Rodolosse A, Campos ML, Rooman I, Lichtenstein M, Real FX

p48, also called Ptf1a (pancreas-specific transcription factor 1a), is a tissue-restricted bHLH (basic helix loop helix) transcription factor which is critical for pancreatic commitment during development and for the activation and maintenance of the acinar differentiation programme in the exocrine pancreas. High-level expression of exocrine digestive enzymes, a hallmark of mature acinar cells, depends largely on the trimeric complex PTF1, ... [more]

Biochem. J. Mar. 01, 2009; 418(2);463-73 [Pubmed: 18834332]

Throughput

Low Throughput

Additional Notes

Figure 2
source of bait and hit not clear

Curated By

BioGRID

Interaction Summary

KAT2A

Gene Ontology Biological Process

Gene Ontology Molecular Function

H3 histone acetyltransferase activity [IDA]histone acetyltransferase activity [IDA]histone deacetylase binding [IPI]protein binding [IPI]transcription coactivator activity [IDA]

Gene Ontology Cellular Component

PTF1A