BAIT

KCNC1

C230009H10Rik, KShIIIB, KV4, Kcr2-1, Kv3.1, NGK2, Shaw
potassium voltage gated channel, Shaw-related subfamily, member 1
GO Process (2)
GO Function (2)
GO Component (4)
Mus musculus
PREY

KCNC1

C230009H10Rik, KShIIIB, KV4, Kcr2-1, Kv3.1, NGK2, Shaw
potassium voltage gated channel, Shaw-related subfamily, member 1
GO Process (2)
GO Function (2)
GO Component (4)
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Differential subcellular localization of the two alternatively spliced isoforms of the Kv3.1 potassium channel subunit in brain.

Ozaita A, Martone ME, Ellisman MH, Rudy B

Voltage-gated K(+) channels containing pore-forming subunits of the Kv3 subfamily have specific roles in the fast repolarization of action potentials and enable neurons to fire repetitively at high frequencies. Each of the four known Kv3 genes encode multiple products by alternative splicing of 3' ends resulting in the expression of K(+) channel subunits differing only in their C-terminal sequence. The ... [more]

J. Neurophysiol. Jul. 01, 2002; 88(1);394-408 [Pubmed: 12091563]

Throughput

  • Low Throughput

Additional Notes

  • Kv3.1a isoform interacts with Kv3.1b isoform

Curated By

  • BioGRID