NEUROD1
Gene Ontology Biological Process
- amacrine cell differentiation [ISS]
- cerebellum development [ISS]
- dentate gyrus development [ISS]
- embryonic organ morphogenesis [ISS]
- endocrine pancreas development [ISS, TAS]
- enteroendocrine cell differentiation [ISS]
- glucose homeostasis [ISS]
- inner ear development [ISS]
- insulin secretion [IDA]
- negative regulation of type B pancreatic cell apoptotic process [ISS]
- neurogenesis [TAS]
- nitric oxide mediated signal transduction [IDA]
- positive regulation of apoptotic process [ISS]
- positive regulation of cell differentiation [ISS]
- positive regulation of neuron differentiation [ISS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription regulatory region DNA binding [IDA]
- positive regulation of transcription, DNA-templated [ISS]
- regulation of cell cycle arrest [ISS]
- regulation of insulin secretion [IC]
- regulation of intestinal epithelial structure maintenance [ISS]
- response to glucose [IMP]
Gene Ontology Molecular Function- E-box binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II transcription coactivator activity [IDA]
- chromatin binding [ISS]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
- E-box binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II transcription coactivator activity [IDA]
- chromatin binding [ISS]
- protein binding [IPI]
- protein heterodimerization activity [IDA, IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription coactivator activity [IDA]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
PDHX
Gene Ontology Biological Process
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Insulin gene transcription is mediated by interactions between the p300 coactivator and PDX-1, BETA2, and E47.
Pancreatic beta-cell-type-specific expression of the insulin gene requires both ubiquitous and cell-enriched activators, which are organized within the enhancer region into a network of protein-protein and protein-DNA interactions to promote transcriptional synergy. Protein-protein-mediated communication between DNA-bound activators and the RNA polymerase II transcriptional machinery is inhibited by the adenovirus E1A protein as a result of E1A's binding to the p300 ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 7
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PDHX NEUROD1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 1055586 |
Curated By
- BioGRID