BAIT

HIST3H3

H3.4, H3/g, H3FT, H3t
histone cluster 3, H3
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

GATAD1

CMD2B, ODAG, RG083M05.2, tcag7.279
GATA zinc finger domain containing 1
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens

Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers.

Vermeulen M, Eberl HC, Matarese F, Marks H, Denissov S, Butter F, Lee KK, Olsen JV, Hyman AA, Stunnenberg HG, Mann M

Trimethyl-lysine (me3) modifications on histones are the most stable epigenetic marks and they control chromatin-mediated regulation of gene expression. Here, we determine proteins that bind these marks by high-accuracy, quantitative mass spectrometry. These chromatin "readers" are assigned to complexes by interaction proteomics of full-length BAC-GFP-tagged proteins. ChIP-Seq profiling identifies their genomic binding sites, revealing functional properties. Among the main findings, ... [more]

Cell Sep. 17, 2010; 142(6);967-80 [Pubmed: 20850016]

Throughput

  • High Throughput

Additional Notes

  • H3K4me3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HIST3H3 GATAD1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2786862

Curated By

  • BioGRID