Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Differential G protein subunit expression by prostate cancer cells and their interaction with CXCR5.

El-Haibi CP, Sharma P, Singh R, Gupta P, Taub DD, Singh S, Lillard JW

Prostate cancer (PCa) cell lines and tissues differentially express CXCR5, which positively correlate with PCa progression, and mediate PCa cell migration and invasion following interaction with CXCL13. However, the differential expression of G protein α, β, and γ subunits by PCa cell lines and the precise combination of these proteins with CXCR5 has not been elucidated.We examined differences in G ... [more]

Mol. Cancer Jun. 19, 2013; 12(0);64 [Pubmed: 23773523]

Throughput

  • Low Throughput

Additional Notes

  • Figure 2C

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GNA13 CXCR5
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
1056899

Curated By

  • BioGRID