TRAF6
Gene Ontology Biological Process
- I-kappaB kinase/NF-kappaB signaling [IMP]
- JNK cascade [ISO]
- T cell receptor signaling pathway [ISO]
- T-helper 1 type immune response [IMP]
- activation of NF-kappaB-inducing kinase activity [ISO]
- activation of protein kinase activity [ISO]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [IMP]
- bone remodeling [IMP]
- bone resorption [IMP]
- cell development [IMP]
- cellular response to lipopolysaccharide [ISO]
- cytokine-mediated signaling pathway [ISO]
- immune response [IMP]
- interleukin-1-mediated signaling pathway [IMP, ISO]
- myeloid dendritic cell differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- neural tube closure [IMP]
- odontogenesis of dentin-containing tooth [IMP]
- organ morphogenesis [IMP]
- ossification [IMP]
- osteoclast differentiation [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IGI, IMP, ISO]
- positive regulation of JUN kinase activity [ISO]
- positive regulation of NF-kappaB transcription factor activity [IMP, ISO]
- positive regulation of T cell cytokine production [ISO]
- positive regulation of T cell proliferation [IMP]
- positive regulation of interleukin-12 biosynthetic process [IMP]
- positive regulation of interleukin-2 production [ISO]
- positive regulation of interleukin-6 biosynthetic process [IMP]
- positive regulation of lipopolysaccharide-mediated signaling pathway [IMP]
- positive regulation of osteoclast differentiation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription regulatory region DNA binding [ISO]
- protein K63-linked ubiquitination [IDA, ISO]
- protein autoubiquitination [ISO, TAS]
- protein complex assembly [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IDA, IGI, IMP]
- regulation of immunoglobulin secretion [IDA]
- response to interleukin-1 [ISO]
- signal transduction [IDA, TAS]
Gene Ontology Molecular Function- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
Gene Ontology Cellular Component
TRAF6
Gene Ontology Biological Process
- I-kappaB kinase/NF-kappaB signaling [IMP]
- JNK cascade [ISO]
- T cell receptor signaling pathway [ISO]
- T-helper 1 type immune response [IMP]
- activation of NF-kappaB-inducing kinase activity [ISO]
- activation of protein kinase activity [ISO]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [IMP]
- bone remodeling [IMP]
- bone resorption [IMP]
- cell development [IMP]
- cellular response to lipopolysaccharide [ISO]
- cytokine-mediated signaling pathway [ISO]
- immune response [IMP]
- interleukin-1-mediated signaling pathway [IMP, ISO]
- myeloid dendritic cell differentiation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- neural tube closure [IMP]
- odontogenesis of dentin-containing tooth [IMP]
- organ morphogenesis [IMP]
- ossification [IMP]
- osteoclast differentiation [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IGI, IMP, ISO]
- positive regulation of JUN kinase activity [ISO]
- positive regulation of NF-kappaB transcription factor activity [IMP, ISO]
- positive regulation of T cell cytokine production [ISO]
- positive regulation of T cell proliferation [IMP]
- positive regulation of interleukin-12 biosynthetic process [IMP]
- positive regulation of interleukin-2 production [ISO]
- positive regulation of interleukin-6 biosynthetic process [IMP]
- positive regulation of lipopolysaccharide-mediated signaling pathway [IMP]
- positive regulation of osteoclast differentiation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [ISO]
- positive regulation of smooth muscle cell proliferation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription regulatory region DNA binding [ISO]
- protein K63-linked ubiquitination [IDA, ISO]
- protein autoubiquitination [ISO, TAS]
- protein complex assembly [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [IDA, IGI, IMP]
- regulation of immunoglobulin secretion [IDA]
- response to interleukin-1 [ISO]
- signal transduction [IDA, TAS]
Gene Ontology Molecular Function- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
- histone deacetylase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase B binding [ISO]
- protein kinase binding [ISO]
- signal transducer activity [TAS]
- thioesterase binding [ISO]
- tumor necrosis factor receptor binding [ISO]
- ubiquitin conjugating enzyme binding [ISO]
- ubiquitin protein ligase activity [IDA, IMP]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Substrate modification with lysine 63-linked ubiquitin chains through the UBC13-UEV1A ubiquitin-conjugating enzyme.
Protein modification with lysine 63-linked ubiquitin chains has been implicated in the non-proteolytic regulation of signaling pathways. To understand the molecular mechanisms underlying this process, we have developed an in vitro system to examine the activity of the ubiquitin-conjugating enzyme UBC13-UEV1A with TRAF6 in which TRAF6 serves as both a ubiquitin ligase and substrate for modification. Although TRAF6 potently stimulates ... [more]
Throughput
- Low Throughput
Additional Notes
- E2 was Ubch5c
- Figure 2
- In vitro reaction
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TRAF6 TRAF6 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| TRAF6 TRAF6 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID