RNF34
Gene Ontology Biological Process
- negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis [IMP]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- negative regulation of signal transduction by p53 class mediator [IMP]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA, IMP]
- protein K48-linked ubiquitination [IMP]
- protein ubiquitination [IDA, IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of oxygen metabolic process [ISS]
Gene Ontology Molecular Function
CASP10
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth.
The destruction of cellular targets during apoptosis is carried out by caspases, which are negatively regulated by the inhibitor of apoptosis proteins (IAP); however, death effector domain (DED) caspases of the extrinsic pathway are refractory to the IAP family. We have isolated a family of apoptotic inhibitors [caspases-8- and -10-associated RING proteins (CARPs)] that bind to and negatively regulate DED ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CASP10 RNF34 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID