Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Linker Histone H1.2 cooperates with Cul4A and PAF1 to drive H4K31 ubiquitylation-mediated transactivation.

Kim K, Lee B, Kim J, Choi J, Kim JM, Xiong Y, Roeder RG, An W

Increasing evidence suggests that linker histone H1 can influence distinct cellular processes by acting as a gene-specific regulator. However, the mechanistic basis underlying such H1 specificity and whether H1 acts in concert with other chromatin-altering activities remain unclear. Here, we show that one of the H1 subtypes, H1.2, stably interacts with Cul4A E3 ubiquitin ligase and PAF1 elongation complexes and ... [more]

Cell Rep Dec. 26, 2013; 5(6);1690-703 [Pubmed: 24360965]

Throughput

  • High Throughput

Additional Notes

  • MudPIT

Curated By

  • BioGRID