PI4K2A
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- BLOC-1 complex [ISO, ISS]
- cytoplasmic vesicle [IDA]
- dendrite [ISO, ISS]
- early endosome membrane [IDA]
- endosome [IDA]
- exocytic vesicle [IDA]
- growing cell tip [IDA]
- host cell presynaptic membrane [ISO, ISS]
- integral component of plasma membrane [ISO, ISS]
- intracellular membrane-bounded organelle [IDA]
- intrinsic component of membrane [ISO, ISS]
- lysosomal membrane [ISO]
- membrane [ISO]
- membrane raft [IDA, ISO]
- mitochondrion [ISO, ISS]
- neuron projection [IDA, ISO]
- neuronal cell body [IDA, ISO, ISS]
- perikaryon [IDA]
- plasma membrane [IDA]
- presynaptic membrane [ISO, ISS]
- protein complex [IDA]
- synaptic vesicle membrane [IDA]
ITCH
Gene Ontology Biological Process
- negative regulation of JNK cascade [ISO]
- negative regulation of NF-kappaB transcription factor activity [ISO]
- negative regulation of alpha-beta T cell proliferation [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of defense response to virus [ISO]
- positive regulation of T cell anergy [ISO]
- positive regulation of protein catabolic process [ISO]
- protein K29-linked ubiquitination [ISO]
- protein K48-linked ubiquitination [ISO]
- protein K63-linked ubiquitination [ISO]
- protein polyubiquitination [ISO]
- protein ubiquitination [ISO]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA, ISO]
- regulation of protein deubiquitination [ISO]
- ubiquitin-dependent protein catabolic process [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Phosphatidylinositol 4-kinase IIα function at endosomes is regulated by the ubiquitin ligase Itch.
Phosphatidylinositol (PI) 4-phosphate (PI(4)P) and its metabolizing enzymes serve important functions in cell signalling and membrane traffic. PI 4-kinase type IIα (PI4KIIα) regulates Wnt signalling, endosomal sorting of signalling receptors, and promotes adaptor protein recruitment to endosomes and the trans-Golgi network. Here we identify the E3 ubiquitin ligase Itch as binding partner and regulator of PI4KIIα function. Itch directly associates ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 2B
Curated By
- BioGRID