BAIT
PI4K2A
Pi4k2
phosphatidylinositol 4-kinase type 2 alpha
GO Process (3)
GO Function (5)
GO Component (22)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- BLOC-1 complex [ISO]
- cytoplasmic vesicle [ISO]
- dendrite [IDA]
- early endosome membrane [ISO]
- endosome [ISO]
- exocytic vesicle [ISO]
- growing cell tip [ISO]
- host cell presynaptic membrane [IDA]
- integral component of plasma membrane [ISO]
- intracellular membrane-bounded organelle [ISO]
- intrinsic component of membrane [ISO]
- lysosomal membrane [ISO]
- membrane [ISO]
- membrane raft [ISO]
- mitochondrion [IDA]
- neuron projection [IDA, ISO]
- neuronal cell body [IDA, ISO]
- perikaryon [ISO]
- plasma membrane [ISO]
- presynaptic membrane [IDA]
- protein complex [ISO]
- synaptic vesicle membrane [ISO]
Mus musculus
PREY
NEDD4
Nedd4a
neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
GO Process (35)
GO Function (12)
GO Component (13)
Gene Ontology Biological Process
- T cell activation [ISO]
- adaptive immune response [ISO]
- blood vessel morphogenesis [ISO]
- cellular response to UV [ISO]
- development involved in symbiotic interaction [ISO]
- endocardial cushion development [ISO]
- glucocorticoid receptor signaling pathway [ISO]
- immune response [IEP]
- lysosomal transport [ISO]
- negative regulation of sodium ion transport [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage [ISO]
- negative regulation of vascular endothelial growth factor receptor signaling pathway [ISO, ISS]
- neuromuscular junction development [ISO]
- neuron projection development [ISO]
- outflow tract morphogenesis [ISO]
- positive regulation of nucleocytoplasmic transport [ISO]
- positive regulation of phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of protein catabolic process [ISO]
- progesterone receptor signaling pathway [ISO]
- protein K63-linked ubiquitination [IDA]
- protein monoubiquitination [ISO]
- protein targeting to lysosome [ISO]
- protein ubiquitination [ISO, ISS]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA, ISO]
- receptor catabolic process [ISO]
- receptor internalization [ISO]
- regulation of dendrite morphogenesis [IDA, ISO]
- regulation of ion transmembrane transport [ISO]
- regulation of membrane potential [ISO]
- regulation of potassium ion transmembrane transporter activity [ISO]
- regulation of synapse organization [ISO]
- response to denervation involved in regulation of muscle adaptation [IEP]
- transmission of virus [ISO]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [ISO]
Gene Ontology Molecular Function- RNA polymerase binding [ISO]
- beta-2 adrenergic receptor binding [ISO]
- phosphoserine binding [ISO]
- phosphothreonine binding [ISO]
- proline-rich region binding [ISO]
- protein C-terminus binding [IDA, IPI]
- protein binding [IPI]
- protein domain specific binding [ISO]
- sodium channel inhibitor activity [ISO]
- ubiquitin binding [ISO]
- ubiquitin protein ligase activity [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
- RNA polymerase binding [ISO]
- beta-2 adrenergic receptor binding [ISO]
- phosphoserine binding [ISO]
- phosphothreonine binding [ISO]
- proline-rich region binding [ISO]
- protein C-terminus binding [IDA, IPI]
- protein binding [IPI]
- protein domain specific binding [ISO]
- sodium channel inhibitor activity [ISO]
- ubiquitin binding [ISO]
- ubiquitin protein ligase activity [ISO]
- ubiquitin-protein transferase activity [IDA, ISO]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Phosphatidylinositol 4-kinase IIα function at endosomes is regulated by the ubiquitin ligase Itch.
Phosphatidylinositol (PI) 4-phosphate (PI(4)P) and its metabolizing enzymes serve important functions in cell signalling and membrane traffic. PI 4-kinase type IIα (PI4KIIα) regulates Wnt signalling, endosomal sorting of signalling receptors, and promotes adaptor protein recruitment to endosomes and the trans-Golgi network. Here we identify the E3 ubiquitin ligase Itch as binding partner and regulator of PI4KIIα function. Itch directly associates ... [more]
EMBO Rep. Dec. 01, 2012; 13(12);1087-94 [Pubmed: 23146885]
Throughput
- Low Throughput
Additional Notes
- Figure S1C
Curated By
- BioGRID