Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Ubiquitin Specific Peptidase 8 (USP8) Regulates Endosomal Trafficking of the Epithelial Na+ Channel.

Zhou R, Tomkovicz VR, Butler PL, Ochoa LA, Peterson ZJ, Snyder PM

Ubiquitination plays a key role in trafficking of the epithelial Na(+) channel ENaC. Previous work indicates that ubiquitination enhances ENaC endocytosis and sorting to lysosomes for degradation. Moreover, a defect in ubiquitination causes Liddle's syndrome, an inherited form of hypertension. In the current work, we identified a role for ubiquitin specific peptidase 8 (USP8) in the control of ENaC ubiquitination ... [more]

J. Biol. Chem. Jan. 07, 2013; 0(0); [Pubmed: 23297398]

Throughput

  • Low Throughput

Additional Notes

  • Figure 5B

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
USP8 SCNN1B
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
1057465

Curated By

  • BioGRID