Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Identifying biological pathways that underlie primordial short stature using network analysis.

Hanson D, Stevens A, Murray PG, Black GC, Clayton PE

Mutations in CUL7, OBSL1 and CCDC8, leading to disordered ubiquitination, cause one of the commonest primordial growth disorders, 3-M syndrome. This condition is associated with (1) abnormal p53 function, (2) GH and/or IGF1 resistance, which may relate to failure to recycle signalling molecules, and (3) cellular IGF2 deficiency. However the exact molecular mechanisms that may link these abnormalities generating growth ... [more]

J. Mol. Endocrinol. Apr. 07, 2014; 0(0); [Pubmed: 24711643]

Throughput

  • High Throughput

Curated By

  • BioGRID