An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Quantitative assessment of complex formation of nuclear-receptor accessory proteins.

Graumann K, Jungbauer A

Like other nuclear receptors, steroid hormone receptors form large protein hetero-complexes in their inactive, ligand-friendly state. Several heat-shock proteins, immunophilins and others have been identified as members of these highly dynamic complexes. The interaction kinetics and dynamics of hsp90, hsp70, p60 (Hop), FKBP52, FKBP51, p48 (Hip) and p23 have been assessed by a biosensor approach measuring the complex formation in ... [more]

Biochem. J. Feb. 01, 2000; 345(0);627-36 [Pubmed: 10642522]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

FKBP4

Gene Ontology Biological Process

Gene Ontology Molecular Function

FK506 binding [IBA, TAS]heat shock protein binding [IPI]peptidyl-prolyl cis-trans isomerase activity [IDA]poly(A) RNA binding [IDA]protein binding [IPI]protein binding, bridging [TAS]

Gene Ontology Cellular Component

HSPA4