Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Quantitative assessment of complex formation of nuclear-receptor accessory proteins.

Graumann K, Jungbauer A

Like other nuclear receptors, steroid hormone receptors form large protein hetero-complexes in their inactive, ligand-friendly state. Several heat-shock proteins, immunophilins and others have been identified as members of these highly dynamic complexes. The interaction kinetics and dynamics of hsp90, hsp70, p60 (Hop), FKBP52, FKBP51, p48 (Hip) and p23 have been assessed by a biosensor approach measuring the complex formation in ... [more]

Biochem. J. Feb. 01, 2000; 345(0);627-36 [Pubmed: 10642522]

Throughput

  • Low Throughput

Curated By

  • BioGRID