An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Gaf-1, a gamma -SNAP-binding protein associated with the mitochondria.

Chen D, Xu W, He P, Medrano EE, Whiteheart SW

The role of alpha/beta-SNAP (Soluble NSF Attachment Protein) in vesicular trafficking is well established; however, the function of the ubiquitously expressed gamma-SNAP remains unclear. To further characterize the cellular role of this enigmatic protein, a two-hybrid screen was used to identify new, gamma-SNAP-binding proteins and to uncover potentially novel functions for gamma-SNAP. One such SNAP-binding protein, termed Gaf-1 (gamma-SNAP associate ... [more]

J. Biol. Chem. Apr. 20, 2001; 276(16);13127-35 [Pubmed: 11278501]

Throughput

Low Throughput

Additional Notes

Figure 2

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAB11FIP5 NAPG	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9991	BioGRID	3098939
NAPG RAB11FIP5	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Chen D (2001)	Low	-	BioGRID	1059565
NAPG RAB11FIP5	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Chen D (2001)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RAB11FIP5

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [IBA]gamma-tubulin binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

NAPG

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]soluble NSF attachment protein activity [IBA]syntaxin binding [IBA]