SQSTM1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PRKCI
Gene Ontology Biological Process
- Golgi vesicle budding [IDA]
- actin filament organization [ISO]
- cell migration [IMP]
- cell-cell junction organization [ISO]
- cellular protein localization [IMP]
- cellular response to insulin stimulus [IMP, ISO]
- establishment of apical/basal cell polarity [ISO]
- eye photoreceptor cell development [ISO]
- negative regulation of glial cell apoptotic process [ISO, ISS]
- negative regulation of neuron apoptotic process [ISO, ISS]
- positive regulation of NF-kappaB transcription factor activity [ISO, ISS]
- positive regulation of endothelial cell apoptotic process [ISO, ISS]
- positive regulation of establishment of protein localization to plasma membrane [ISO]
- positive regulation of glial cell proliferation [ISO, ISS]
- positive regulation of glucose import [ISO]
- positive regulation of neuron projection development [ISO, ISS]
- protein phosphorylation [IDA, ISO]
- response to interleukin-1 [IMP]
- response to peptide hormone [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [IDA]
- Schmidt-Lanterman incisure [ISO]
- apical part of cell [ISO]
- apical plasma membrane [ISO]
- cell leading edge [IDA]
- cytoplasm [ISO]
- cytosol [IDA, ISO, ISS]
- extracellular vesicular exosome [ISO]
- intercellular bridge [ISO]
- microtubule cytoskeleton [ISO]
- nucleus [ISO, ISS]
- plasma membrane [IDA]
- protein complex [IDA]
- tight junction [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Nerve growth factor stimulates the interaction of ZIP/p62 with atypical protein kinase C and targets endosomal localization: evidence for regulation of nerve growth factor-induced differentiation.
Atypical protein kinase Cs zeta and lambda/iota play a functional role in the regulation of NGF-induced differentiation and survival of pheochromocytoma, PC12 cells [Coleman and Wooten, 1994; Wooten et al., 1999]. Here we demonstrate an NGF-dependent interaction of aPKC with its binding protein, ZIP/p62. Although, ZIP/p62 was not a PKC-iota substrate, the formation of a ZIP/p62-aPKC complex in PC12 cells ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SQSTM1 PRKCI | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID