Biochemical Activity (Nedd(Rub1)ylation)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.

Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA

Ubiquitin and ubiquitin-like proteins (UBLs) are directed to targets by cascades of E1, E2, and E3 enzymes. The largest ubiquitin E3 subclass consists of cullin-RING ligases (CRLs), which contain one each of several cullins (CUL1, -2, -3, -4, or -5) and RING proteins (RBX1 or -2). CRLs are activated by ligation of the UBL NEDD8 to a conserved cullin lysine. ... [more]

Mol. Cell Feb. 27, 2009; 33(4);483-95 [Pubmed: 19250909]

Throughput

  • Low Throughput

Additional Notes

  • NEDD8 transthiolation
  • UBE2M and UBE2F are charged with thioester linked Nedd8 by NAE1-UBA3~NEDD8 in vitro

Curated By

  • BioGRID