Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

N-myristoyltransferase 2 expression in human colon cancer: cross-talk between the calpain and caspase system.

Selvakumar P, Smith-Windsor E, Bonham K, Sharma RK

A number of viral and eukaryotic proteins which undergo a lipophilic modification by the enzyme N-myristoyltransferase (NMT: NMT1 and NMT2) are required for signal transduction and regulatory functions. To investigate whether NMT2 contributes to the pathogenesis of colorectal carcinoma, we observed a higher expression of NMT2 in most of the cases of cancerous tissues compared to normal tissues (84.6% of ... [more]

FEBS Lett. Apr. 03, 2006; 580(8);2021-6 [Pubmed: 16530191]

Throughput

  • Low Throughput

Curated By

  • BioGRID