Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

ZNF451 is a novel PML body- and SUMO-associated transcriptional coregulator.

Karvonen U, Jaeaeskelaeinen T, Rytinki M, Kaikkonen S, Palvimo JJ

Covalent modification by small ubiquitin-related modifiers (SUMOs) is an important means to regulate dynamic residency of transcription factors within nuclear compartments. Here, we identify a multi-C(2)H(2)-type zinc finger protein (ZNF), ZNF451, as a novel nuclear protein that can be associated with promyelocytic leukemia bodies. In keeping with its interaction with SUMO E2 conjugase Ubc9 and SUMOs, ZNF451 is covalently modified ... [more]

J. Mol. Biol. Oct. 10, 2008; 382(3);585-600 [Pubmed: 18656483]

Throughput

  • Low Throughput

Additional Notes

  • mammalian two-hybrid

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PIAS1 ZNF451
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
1060122

Curated By

  • BioGRID