BAIT

SRP54

SRH1, L000002064, YPR088C
Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
Saccharomyces cerevisiae (S288c)
PREY

OPI3

PEM2, bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase, L000001304, YJR073C
Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

Defining the Specificity of Cotranslationally Acting Chaperones by Systematic Analysis of mRNAs Associated with Ribosome-Nascent Chain Complexes.

Del Alamo M, Hogan DJ, Pechmann S, Albanese V, Brown PO, Frydman J

Polypeptides exiting the ribosome must fold and assemble in the crowded environment of the cell. Chaperones and other protein homeostasis factors interact with newly translated polypeptides to facilitate their folding and correct localization. Despite the extensive efforts, little is known about the specificity of the chaperones and other factors that bind nascent polypeptides. To address this question we present an ... [more]

PLoS Biol. Jul. 01, 2011; 9(7);e1001100 [Pubmed: 21765803]

Throughput

  • High Throughput

Additional Notes

  • immunoaffinity isolation of Srp54-TAP along with its cotranslational associated RNC-mRNAs complexes to isolate mRNAs encoding nascent proteins specifically recognized by SRP

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
OPI3 SRP54
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.3993BioGRID
589112

Curated By

  • BioGRID