BAIT

SSB2

YG103, Hsp70 family ATPase SSB2, L000002715, YNL209W
Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

RPT1

CIM5, YTA3, proteasome regulatory particle base subunit RPT1, L000002557, YKL145W
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
UPS Project
Saccharomyces cerevisiae (S288c)

Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

The cotranslational function of ribosome-associated Hsp70 in eukaryotic protein homeostasis.

Willmund F, del Alamo M, Pechmann S, Chen T, Albanese V, Dammer EB, Peng J, Frydman J

In eukaryotic cells a molecular chaperone network associates with translating ribosomes, assisting the maturation of emerging nascent polypeptides. Hsp70 is perhaps the major eukaryotic ribosome-associated chaperone and the first reported to bind cotranslationally to nascent chains. However, little is known about the underlying principles and function of this interaction. Here, we use a sensitive and global approach to define the ... [more]

Cell Jan. 17, 2013; 152(1);196-209 [Pubmed: 23332755]

Throughput

  • High Throughput

Additional Notes

  • isolation of cotranslationally bound SSB-RNC complexes allows us to identify the chaperone-bound nascent polypeptides through analysis of the mRNAs encoding the substrates

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SSB2 RPT1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1373BioGRID
2065618

Curated By

  • BioGRID