Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Protein interaction screening for the ankyrin repeats and suppressor of cytokine signaling (SOCS) box (ASB) family identify Asb11 as a novel endoplasmic reticulum resident ubiquitin ligase.

Andresen CA, Smedegaard S, Sylvestersen KB, Svensson C, Iglesias-Gato D, Cazzamali G, Nielsen TK, Nielsen ML, Flores-Morales A

The ankyrin and SOCS (suppressor of cytokine signaling) box (ASB) family of proteins function as the substrate recognition subunit in a subset of Elongin-Cullin-SOCS (ECS) E3 ubiquitin ligases. Despite counting 18 members in humans, the identity of the physiological targets of the Asb proteins remains largely unexplored. To increase our understanding of the function of ASB proteins, we conducted a ... [more]

J. Biol. Chem. Jan. 24, 2014; 289(4);2043-54 [Pubmed: 24337577]

Throughput

Low Throughput

Additional Notes

Duo-link PLA

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ASB11 CUL5	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Chen FY (2019)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ASB11

CUL5

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium channel activity [TAS]protein binding [IPI]receptor activity [TAS]ubiquitin protein ligase binding [IDA]

Gene Ontology Cellular Component

Co-localization