An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The central role of EED in the orchestration of polycomb group complexes.

Cao Q, Wang X, Zhao M, Yang R, Malik R, Qiao Y, Poliakov A, Yocum AK, Li Y, Chen W, Cao X, Jiang X, Dahiya A, Harris C, Feng FY, Kalantry S, Qin ZS, Dhanasekaran SM, Chinnaiyan AM

Polycomb repressive complexes 1 and 2 (PRC1 and 2) play a critical role in the epigenetic regulation of transcription during cellular differentiation, stem cell pluripotency and neoplastic progression. Here we show that the polycomb group protein EED, a core component of PRC2, physically interacts with and functions as part of PRC1. Components of PRC1 and PRC2 compete for EED binding. ... [more]

Nat Commun Jan. 24, 2014; 5(0);3127 [Pubmed: 24457600]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

BMI1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]protein binding [IPI]zinc ion binding [IDA]

Gene Ontology Cellular Component

TIMM50

Gene Ontology Biological Process

Gene Ontology Molecular Function

interleukin-2 receptor binding [IDA]phosphoprotein phosphatase activity [IDA]protein binding [IPI]protein serine/threonine phosphatase activity [IDA]protein tyrosine phosphatase activity [IDA]ribonucleoprotein complex binding [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

The central role of EED in the orchestration of polycomb group complexes.

Throughput

Curated By

RING-like zinc finger domain binding [IPI]
protein binding [IPI]
zinc ion binding [IDA]

interleukin-2 receptor binding [IDA]
phosphoprotein phosphatase activity [IDA]
protein binding [IPI]
protein serine/threonine phosphatase activity [IDA]
protein tyrosine phosphatase activity [IDA]
ribonucleoprotein complex binding [IDA]