BAIT

UCHL3

UCH-L3, RP11-173B14.3
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
UPS Project
GO Process (1)
GO Function (4)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

NEDD8

NEDD-8
neural precursor cell expressed, developmentally down-regulated 8
UPS Project
Homo sapiens

Biochemical Activity (Proteolytic Processing)

An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Cleavage of the C-terminus of NEDD8 by UCH-L3.

Wada H, Kito K, Caskey LS, Yeh ET, Kamitani T

NEDD8 is a novel ubiquitin-like protein that has been shown to conjugate to nuclear proteins in a manner analogous to ubiquitination and sentrinization. To identify proteins that are involved in the NEDD8-conjugation and de-conjugation pathway, the yeast two-hybrid system was used to screen a human heart cDNA library using NEDD8 as a bait. Seven strongly positive clones were found to ... [more]

Biochem. Biophys. Res. Commun. Oct. 29, 1998; 251(3);688-92 [Pubmed: 9790970]

Throughput

  • Low Throughput

Additional Notes

  • UCHL3 processed the 3' end of NEDD8 in vitro

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NEDD8 UCHL3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
675484
NEDD8 UCHL3
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
1067215
NEDD8 UCHL3
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
282736
NEDD8 UCHL3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
282737

Curated By

  • BioGRID