UBE2I
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- cellular protein modification process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- post-translational protein modification [TAS]
- protein sumoylation [IDA, TAS]
- protein ubiquitination [IBA]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PPARA
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- circadian regulation of gene expression [ISS]
- fatty acid transport [TAS]
- gene expression [TAS]
- intracellular receptor signaling pathway [IDA]
- lipid metabolic process [TAS]
- negative regulation of appetite [ISS]
- negative regulation of cholesterol storage [IDA]
- negative regulation of glycolytic process [IC]
- negative regulation of macrophage derived foam cell differentiation [IC, IDA]
- negative regulation of receptor biosynthetic process [IDA]
- negative regulation of sequestering of triglyceride [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of fatty acid beta-oxidation [TAS]
- positive regulation of fatty acid oxidation [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of circadian rhythm [ISS]
- regulation of glycolytic by positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [IDA]
- small molecule metabolic process [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- drug binding [IDA]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- lipid binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [ISS]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- steroid hormone receptor activity [IDA]
- ubiquitin conjugating enzyme binding [IPI]
- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- drug binding [IDA]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- lipid binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [ISS]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- steroid hormone receptor activity [IDA]
- ubiquitin conjugating enzyme binding [IPI]
Gene Ontology Cellular Component
- nucleoplasm [TAS]
- nucleus [TAS]
Biochemical Activity (Sumoylation)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
SUMOylation of human peroxisome proliferator-activated receptor alpha inhibits its trans-activity through the recruitment of the nuclear corepressor NCoR.
The nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha) is a key regulator of genes implicated in lipid homeostasis and inflammation. PPARalpha trans-activity is enhanced by recruitment of coactivators such as SRC1 and CBP/p300 and is inhibited by binding of corepressors such as NCoR and SMRT. In addition to ligand binding, PPARalpha activity is regulated by post-translational modifications such as phosphorylation ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 2
- source of enzymes: SUMOlink kit
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBE2I PPARA | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 590051 |
Curated By
- BioGRID