An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Defining human ERAD networks through an integrative mapping strategy.

Christianson JC, Olzmann JA, Shaler TA, Sowa ME, Bennett EJ, Richter CM, Tyler RE, Greenblatt EJ, Harper JW, Kopito RR

Proteins that fail to correctly fold or assemble into oligomeric complexes in the endoplasmic reticulum (ER) are degraded by a ubiquitin- and proteasome-dependent process known as ER-associated degradation (ERAD). Although many individual components of the ERAD system have been identified, how these proteins are organized into a functional network that coordinates recognition, ubiquitylation and dislocation of substrates across the ER ... [more]

Nat. Cell Biol. Jan. 01, 2012; 14(1);93-105 [Pubmed: 22119785]

Throughput

High Throughput

Additional Notes

in the presence of TX100
in the presence of digitonin

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FAF2 NPEPPS	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Raman M (2015)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FAF2

Gene Ontology Biological Process

Gene Ontology Molecular Function

lipase binding [IDA]lipase inhibitor activity [IDA]protein binding [IPI]ubiquitin binding [IDA]ubiquitin protein ligase binding [IDA]

Gene Ontology Cellular Component

NPEPPS