Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Association of the von Hippel-Lindau protein with AUF1 and post-transcriptional regulation of Vascular Endothelial Growth Factor A mRNA.

Xin H, Brown JA, Gong C, Fan H, Brewer G, Gnarra JR

The von Hippel-Lindau (VHL) tumor suppressor gene product is the recognition component of an E3 ubiquitin ligase and is inactivated in patients with VHL disease and in most sporadic clear cell renal carcinomas (RCC). pVHL controls oxygen-responsive gene expression at the transcriptional and post-transcriptional levels. The vascular endothelial growth factor A (VEGFA) mRNA contains AU-rich elements (AREs) in the 3' ... [more]

Unknown Nov. 15, 2011; 0(0); [Pubmed: 22086907]

Throughput

  • Low Throughput

Additional Notes

  • Figure 2B

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CUL2 HNRNPD
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
688103

Curated By

  • BioGRID