BAIT

SUMO2

HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A
small ubiquitin-like modifier 2
Homo sapiens
PREY

TANK

I-TRAF, ITRAF, TRAF2
TRAF family member-associated NFKB activator
GO Process (2)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Inducible SUMO modification of TANK alleviates its repression of TLR7 signalling.

Renner F, Saul VV, Pagenstecher A, Wittwer T, Schmitz ML

Adaptor proteins allow temporal and spatial coordination of signalling. In this study, we show SUMOylation of the adaptor protein TANK and its interacting kinase TANK-binding kinase 1 (TBK1). Modification of TANK by the small ubiquitin-related modifier (SUMO) at the evolutionarily conserved Lys 282 is triggered by the kinase activities of IκB kinase ɛ (IKKɛ) and TBK1. Stimulation of TLR7 leads ... [more]

EMBO Rep. Feb. 01, 2011; 12(2);129-35 [Pubmed: 21212807]

Throughput

  • Low Throughput

Additional Notes

  • #LPTM
  • Likely post-translational modification

Curated By

  • BioGRID