BAIT
SUMO2
HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A
small ubiquitin-like modifier 2
GO Process (4)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
NOP58
NOP5, NOP5/NOP58, HSPC120
NOP58 ribonucleoprotein
GO Process (4)
GO Function (3)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
Reversible protein modification by small ubiquitin-like modifiers (SUMOs) is critical for eukaryotic life. Mass spectrometry-based proteomics has proven effective at identifying hundreds of potential SUMO target proteins. However, direct identification of SUMO acceptor lysines in complex samples by mass spectrometry is still very challenging. We have developed a generic method for the identification of SUMO acceptor lysines in target proteins. ... [more]
Mol. Cell Aug. 27, 2010; 39(4);641-52 [Pubmed: 20797634]
Throughput
- Low Throughput
Curated By
- BioGRID