UBE2B
Gene Ontology Biological Process
- DNA repair [IGI]
- canonical Wnt signaling pathway [ISS]
- cellular response to DNA damage stimulus [IDA]
- histone H2A ubiquitination [IMP]
- negative regulation of cAMP-mediated signaling [IDA]
- postreplication repair [IDA, NAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K11-linked ubiquitination [IDA]
- protein K48-linked ubiquitination [IDA]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA]
- protein monoubiquitination [IMP]
- protein polyubiquitination [IMP]
- protein stabilization [IMP]
- protein ubiquitination [IDA]
- response to UV [IGI]
- response to drug [IDA]
- spermatogenesis [TAS]
- ubiquitin-dependent protein catabolic process [IDA, NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CBX5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
RAD6 promotes homologous recombination repair by activating the autophagy-mediated degradation of heterochromatin protein HP1.
Efficient DNA double-strand break (DSB) repair is critical for the maintenance of genome stability. Unrepaired or mis-repaired DSBs cause chromosomal rearrangements that can result in severe consequences such as tumorigenesis. RAD6 is an E2 ubiquitin-conjugating enzyme that plays a pivotal role in repairing UV-induced DNA damage. Here, we present evidence that RAD6 is also required for DNA DSB repair via ... [more]
Throughput
- Low Throughput
Additional Notes
- Data not shown
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| UBE2B CBX5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CBX5 UBE2B | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CBX5 UBE2B | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID