Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Assessment of Rab11-FIP2 interacting proteins in vitro.

Ducharme NA, Jin M, Lapierre LA, Goldenring JR

Members of the Rab family of small GTPases are involved in multiple trafficking events in both endocytotic and biosynthetic pathways. To understand more fully the regulation of these events, a concerted effort is underway to ascertain the binding partners and regulators of Rabs. Here, we describe methods to assess binding of Rab11a with Rab11-FIP2 and other Rab11-FIPs utilizing a modified ... [more]

Meth. Enzymol. Feb. 14, 2006; 403(0);706-15 [Pubmed: 16473632]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MYO5B RAB11FIP2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
RAB11FIP2 MYO5B
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID