Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interactions between EHD proteins and Rab11-FIP2: a role for EHD3 in early endosomal transport.

Naslavsky N, Rahajeng J, Sharma M, Jovic M, Caplan S

Eps15 homology domain (EHD) 1 enables membrane recycling by controlling the exit of internalized molecules from the endocytic recycling compartment (ERC) en route to the plasma membrane, similar to the role described for Rab11. However, no physical or functional connection between Rab11 and EHD-family proteins has been demonstrated yet, and the mode by which they coordinate their regulatory activity remains ... [more]

Mol. Biol. Cell Jan. 01, 2006; 17(1);163-77 [Pubmed: 16251358]

Throughput

  • Low Throughput

Curated By

  • BioGRID