An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release.

Yao I, Takagi H, Ageta H, Kahyo T, Sato S, Hatanaka K, Fukuda Y, Chiba T, Morone N, Yuasa S, Inokuchi K, Ohtsuka T, Macgregor GR, Tanaka K, Setou M

Little is known about how synaptic activity is modulated in the central nervous system. We have identified SCRAPPER, a synapse-localized E3 ubiquitin ligase, which regulates neural transmission. SCRAPPER directly binds and ubiquitinates RIM1, a modulator of presynaptic plasticity. In neurons from Scrapper-knockout (SCR-KO) mice, RIM1 had a longer half-life with significant reduction in ubiquitination, indicating that SCRAPPER is the predominant ... [more]

Cell Sep. 07, 2007; 130(5);943-57 [Pubmed: 17803915]

Throughput

Low Throughput

Additional Notes

E2: UbcH5
Figure 2F
NEDD8 system added to reaction
SCF complex containing Skp1 and Cullin1 was immunoprecipitated via SCRAPPER subunit

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FBXL20 RIMS1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Yao I (2007)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FBXL20

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

RIMS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [ISO]SH3 domain binding [ISO]ion channel binding [IDA]poly(A) RNA binding [ISO]protein binding [IPI]protein kinase binding [ISO]

Gene Ontology Cellular Component

Biochemical Activity (Ubiquitination)