BAIT

INSR

CD220, HHF5
insulin receptor
GO Process (28)
GO Function (13)
GO Component (9)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The last enzyme of the de novo purine synthesis pathway ATIC plays a central role in insulin signalling and the Golgi/endosomes protein network.

Boutchueng-Djidjou M, Collard-Simard G, Fortier S, Hebert SS, Kelly I, Landry CR, Faure RL

Insulin is internalized with its cognate receptor into the endosomal apparatus rapidly after binding to hepatocytes. We performed a bioinformatic screen of Golgi/endosome (G/E) hepatic protein fractions and found that ATIC, which is a rate limiting enzyme in the de novo purine biosynthesis pathway, and PTPLAD1 are associated with IR internalisation. The IR interactome (IRGEN) connects ATIC to AMPK within ... [more]

Mol. Cell Proteomics Feb. 16, 2015; 0(0); [Pubmed: 25687571]

Throughput

  • Low Throughput

Curated By

  • BioGRID